Operating the airtight mini-incubator is straightforward. The incubator is placed on the flow cabinet and the valve at the back of the incubator is used to connect to the gas-outlet via snap-lock. Four-well dishes or round culture dishes with gametes are placed on the shelves. With the lid loosely closed a gas flow for around 3 minutes will secure the correct atmosphere in the incubator. By closing the lid and releasing the snap-lock the atmosphere will remain constant in the incubator until the lid is opened. The incubator can now be moved to the main incubator, where disturbances in the atmosphere will not be reflected in the mini-incubator.